rabbit anti ago2  (Sino Biological)

Journal: Nucleic Acids Research

Article Title: Nuclear Argonaute:miRNA complexes recognize target sequences within chromatin-associated RNA and silence gene expression

doi: 10.1093/nar/gkaf800

Figure Lengend Snippet: AGO2 and other protein factors involved in miRNA-mediated regulation can associate with chromatin. Western blots showing ( A ) AGO2 localization and purity of cytoplasm (CY), nucleoplasm (NP), and chromatin (CH) in wild-type samples ( N = 3), ( B ) purity [calnexin for endoplasmic reticulum (ER)] and localization of DROSHA, Dicer, TNRC6A, and AGO1–4 ( N = 3), and ( C ) AGO2 localization in the nuclear localization sequence (NLS)-AGO2 knock-in cell line ( N = 3).

Article Snippet: Western blot was visualized using anti-AGO2 primary antibody (50683-RP02, SinoBiological) at a 1:4000 dilution, with TrueBlot anti-rabbit secondary antibody (18-8816-31, Rockland) at 1:8000 dilution.

Techniques: Western Blot, Sequencing, Knock-In

Journal: Nucleic Acids Research

Article Title: Nuclear Argonaute:miRNA complexes recognize target sequences within chromatin-associated RNA and silence gene expression

doi: 10.1093/nar/gkaf800

Figure Lengend Snippet: AGO2 interacts with target RNAs in all cellular compartments. ( A ) Schematic outlining the chimeric eCLIP protocol used for the identification of AGO2-binding sites. Distribution of reproducible AGO2 ( B ) nonchimeric and ( C ) chimeric binding peaks across genomic features in cytoplasm ( N = 2), whole nucleus ( N = 2), and chromatin ( N = 2) fractions. Chimeric binding peaks only include chimeric reads that include miRNAs that map to the miRGeneDB database . ( D ) Percentage of chimeric miRNAs from the top eight miRNA families sharing the same seed sequence.

Article Snippet: Western blot was visualized using anti-AGO2 primary antibody (50683-RP02, SinoBiological) at a 1:4000 dilution, with TrueBlot anti-rabbit secondary antibody (18-8816-31, Rockland) at 1:8000 dilution.

Techniques: Binding Assay, Sequencing

Journal: Nucleic Acids Research

Article Title: Nuclear Argonaute:miRNA complexes recognize target sequences within chromatin-associated RNA and silence gene expression

doi: 10.1093/nar/gkaf800

Figure Lengend Snippet: HMGA2 RNA is bound by AGO2 in all compartments of the cell. Representative IGV browser images of chimeric eCLIP reads within the 3′-UTR of HMGA2 in the ( A ) cytoplasm, ( B ) nucleus, and ( C ) chromatin. HMGA2 is located on chromosome 12 (chr12). Peak height is defined as read density in reads per million (RPM). All AGO2 peaks are reproducible and meet the following criteria: log2FC > 3 (IP versus input) at least three reads per peak.

Article Snippet: Western blot was visualized using anti-AGO2 primary antibody (50683-RP02, SinoBiological) at a 1:4000 dilution, with TrueBlot anti-rabbit secondary antibody (18-8816-31, Rockland) at 1:8000 dilution.

Techniques:

Journal: bioRxiv

Article Title: Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8

doi: 10.1101/2025.05.28.656609

Figure Lengend Snippet: a. Western blot analyses of Ago2 protein levels in PA-1 WT and two independent DGCR8 KO clones. Tubulin serves as a loading control. b. Scheme representing the pathways targeted by chemical inhibitors used in . c. Western blot analyses of MAVS protein levels in PA-1 WT and two independent DGCR8 KO clones. GAPDH serves as a loading control (left). Quantification of MAVS protein levels using three independent biological replicates (right). Error bars plot mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. P-values are determined by one-sample t-test. The dashed line represents the WT mean. d. IFIT2 expression levels in WT and two DGCR8 KO clones by RT-qPCR, after treatment with lamivudine, emtricitabine or vehicle. Data are normalised to GAPDH and expressed relative to WT mock sample. Error bars plot mean ± SD of two biological replicates.

Article Snippet: Antibodies used include anti-DGCR8 (1:1000, ab90579, Abcam), anti-Drosha (1:1000, NBP1-03349, NovusBio), anti-pEIF2α (1:1000, 3398, Cell Signalling), anti-IFIT3 (1:500, ab76818, Abcam), anti-RIG-I (1:1000, 3743, Cell Signalling), anti-DHX9 (1:1000, 70998 Cell Signalling), anti-ZCCHC8 (1:1000, A301-806 Thermo Fisher Scientific), anti-Ago2 (1:1000, 2897S Cell Signalling), anti-α-tubulin (1:1000, sc-23948, Santa Cruz Biotechnology), anti-vinculin (1:100000, V9131 Sigma Aldrich) anti-β-actin (1:15000, A1978, Sigma-Aldrich), anti-GAPDH (1:1000, CB1001, Sigma Aldrich).

Techniques: Western Blot, Clone Assay, Control, Expressing, Quantitative RT-PCR